Plasmid_Backbone
Part:BBa_K245008
Designed by: Nika Debeljak Group: iGEM09_Slovenia (2009-09-26)
BB-NIC-III-HisN
This plasmid backbone is the same as BBa_K245004 except that the restriction sites AgeI and XmaI are exchanged (which results in NgoMIV and XmaI restriction sites in prefix AgeI and BspEI in suffix). This detail enables extension of part cloned into vector with SG on C-terminal or/and PG on N-terminal.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 2244
Illegal SpeI site found at 13
Illegal PstI site found at 77 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal SpeI site found at 13
Illegal PstI site found at 77
Illegal NotI site found at 70
Illegal NotI site found at 2125 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 2244
Illegal SpeI site found at 13
Illegal PstI site found at 77 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 2244
Illegal SpeI site found at 13
Illegal PstI site found at 77
Illegal NgoMIV site found at 2250
Illegal AgeI site found at 1 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1158
[edit]
Categories
Parameters
None |